Translational_Unit

Part:BBa_K1472612

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-29)

Delta 9,12 and 15 Desaturase

This translational unit is composed of the delta 9, delta 12 and delta 15 desaturase genes with an RBS upstream of each coding sequence. Desaturases introduce cis-carbon double bonds to the carbon chain of fatty acids. ∆9 desaturase introduces a double bond to stearic acid between carbon 9 and carbon 10 to produce oleic acid. ∆12 desaturase introduces a double bond between carbon 12 and carbon 13 of oleic acid so to produce linoleic acid. In the end, ∆15 desaturase converts linoleic acid into alpha-linolenic acid by adding a double bond between carbon 15 and carbon 16 (Figure 1).

CityU HK newBB depmt91215b.jpg

Figure 1. The conversion of stearic acid into alpha-linolenic acid by 3 desaturases.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2095
    Illegal suffix found in sequence at 3198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2095
    Illegal SpeI site found at 3199
    Illegal PstI site found at 3213
    Illegal NotI site found at 2101
    Illegal NotI site found at 3206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2095
    Illegal BglII site found at 1070
    Illegal BamHI site found at 465
    Illegal BamHI site found at 1430
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2095
    Illegal suffix found in sequence at 3199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2095
    Illegal XbaI site found at 2110
    Illegal SpeI site found at 3199
    Illegal PstI site found at 3213
    Illegal AgeI site found at 289
    Illegal AgeI site found at 1251
    Illegal AgeI site found at 1515
    Illegal AgeI site found at 1653
    Illegal AgeI site found at 2508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None